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KMID : 0364820130490030292
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Korean Journal of Microbiology
2013 Volume.49 No. 3 p.292 ~ p.296
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Multiplex Real-Time PCR for Simultaneous Detection of 6 Periodontopathic Bacteria
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Cho Hong-Bum
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Abstract
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This study utilized an analysis method for detecting six microorganisms, such as Actinobacillus actinomycetemcomitans,
Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, and Prevotella
intermedia, triggering periodontal disease, using multiplex real-time polymerase chain reaction (PCR). The analysis
including internal control was made by dividing the six species into two groups using four fluorescence dyes, and it
was verified that there was no interference or cross-reaction between the target species and different kinds of oral
microbial species. Qualitative and quantitative analyses were conducted on each microorganism in various samples,
such as saliva and the plaque, using the multiplex real-time PCR and comparative analysis between periodontitis
patients and healthy people, revealing obvious differences between them.
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KEYWORD
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multiplex real-time PCR, periodontopathic bacteria, plaque, saliva
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